Binary vector pcambia-1300

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August undefined, 2024

WebJul 1, 2024 · The pCAMBIA 1300 plasmid containing a GFP reporter gene driven by the Super promoter and harboring a Hygromycin resistance gene was used for transformation of somatic embryos. ... we transfected embryogenic calli with A. tumefaciens strain GV3101 containing a pCAMBIA 1300 binary vector, ... WebA binary vector was invented soon after it had been elucidated that crown gall tumorigenesis was caused by genetic transformation of plant cells with a piece of DNA, T-DNA for transferred DNA,...

pCambia1281Z Plant Expression Vector

WebNov 25, 2024 · In addition, we tested the pCAMBIA 1300-PtHCA 2-1 promoter-GUS binary expression vector, which contains the GUS gene ORF under the control of Populus … WebJul 24, 2013 · A twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase ( hpt) marker gene. how calories to maintain weight

Transfer DNA binary system - Wikipedia

WebNov 15, 2024 · The pCambia-1300 binary vector was used as a backbone. A pCambia–BSCTV vector was used as template to amplify pV86 or pC86 trans-activated promoters using primers pV86-GUS-R1-F/R or pC86 … WebBinary vector pCAMBIA-1300 NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, … WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … how calories in a cucumber

Stable and Efficient Agrobacterium-Mediated Genetic

Development of a Gateway-compatible pCAMBIA binary vector for …

WebSep 1, 2015 · The pCambia 2300 vector assumes stable genomic insertion and expression in plant species ( Hajdukiewicz et al., 1994) and the proper Gateway®-like function and … how calories in a fried eggWebthe binary vector pCambia 1300-Rb:: uidA to replace the uidA gene and generate pCambia 1300-Rb::MCM2 fragment2. The M2-sense1 primer allowed us to add an XbaI restriction site at the 5 ′ end of the PCR product, and M2-antisense1 was designed downstream of the internal XbaI (1844 bp down-stream of the start codon) site. The … how calories in an orange

"WebSep 8, 2014 · The fragment was then ligated with a GFP-containing pCAMBIA 1300 binary vector to develop the vector named as pDsTGFP (Figure 1B). The vector has two AhdI sites at the 13 th and 1329 th position, respectively (Figure 1B). Thus, the 3′ T-overhang ends can be generated by digesting the pDsTGFP vector with the enzyme AhdI (Figure … " - Binary vector pcambia-1300

Binary vector pcambia-1300

质粒载体pWM1011,pCM184,pGWB403系列 - Biovector

WebBinary vector pCAMBIA 1301 showing hptII gene near left border and GUS gene near right border of T-DNA from publication: An Efficient Protocol for the Agrobacterium-mediated Genetic Transformation ... WebThe cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants.

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WebThe pBIN19 vector was developed in the 1980s and is one of the first and most widely used binary vectors. The pGreen vector, which was developed in 2000, is a newer version of … WebBinary vector pCAMBIA-1301 Taxonomy ID: 118389 (for references in articles please use NCBI:txid118389) current name. Binary vector pCAMBIA-1301. NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, Archaeal and Plant Plastid)

WebpCAMBIA1300 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … WebpCAMBIA1302. Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GFP genes. Sequence Author: Cambia. Open in …

WebSDS for the pCambia vectors are available by request from Marker Gene Technologies, Inc. Shipping and Storage The plant expression vectors are shipped at room temperature. Store all samples of the vector at –20 C or below, once resuspended. Products are guaranteed for six months from date of shipment when stored properly. Contents WebpCAMBIA has wide range of vector like 1300,2300,2301. it is binary vector kanamycin based with small replicon and has plant selection …

WebBinary vector pCAMBIA-1300, complete sequence Binary vector pCAMBIA-1300, complete sequence. Your browsing activity is empty. Activity recording is turned off. Turn …

WebAug 14, 2015 · Grains of an insect resistant transgenic Bt rice (Oryza sativa L. Japonica cv Ariete), transformed by Agrobacterium tumefaciens with the binary vector pCAMBIA 1300 containing the synthetic Cry1B gene from Bacillus thuringiensis, and its corresponding wild-type (WT) were used in this study . The CRM of the GM maize MON863 9.85 % (ERM … how many paper girls books are thereWebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β … how calvinist are youWebEnter the email address you signed up with and we'll email you a reset link. how can 18.2-134 trespass on posted propertyWeb作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。. 通过Google搜索"Biovector+您所需 … how calories turn into fatWebThe vector pCAMBIA- 1300: SniOLP was digested with Eco RI enzyme and made blunt ended by end filling. This blunt-ended vector was used for cloning the blunt-ended Rs-AFP2 expression cassette. how many papers are made from a treeWebClassification and research data for Binary vector pCAMBIA-1300, unclassified. Classification and research data for Binary vector pCAMBIA-1300, unclassified. Skip to … how came my man i\u0027 the stocksWebNov 25, 2024 · Agrobacterium tumefaciens strain GV3101 was transformed with the pBI121, pCAMBIA 1300, and recombinant pCAMBIA-PTHCA 2-1 Promoter-GUS binary vectors ( Supplementary Figure S1 ). The pBI121 … how many panzer 3 were made