WebNov 2, 2024 · Aconitine linoleate (1) is a lipo-diterpenoid alkaloid, isolated from Aconitum sinchiangense W. T. Wang. The study aimed at investigating the anti-proliferative efficacy and the underlying mechanisms of 1 against MCF-7 and MCF-7/ADR cells, as well as obvious the safety evaluation in vivo. The cytotoxic activities of 1 were measured in vitro. … WebJul 21, 2024 · Cells were harvested and proteins were extracted by RIPA buffer (Biotopped, Beijing, China) containing 1% phenylmethanesulfonyl fluoride (PMSF) (Biotopped). Proteins were quantified by Bradford assay, separated by 12% polyacrylamide gel (SDS-PAGE), and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Kenilworth, …
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