Pulk1 s757

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August undefined, 2024

WebMay 19, 2013 · Whole cell lysates were immunoblotted with pULK1 S757 (mTORC1-target site), pS6K and ULK1 antibodies (bottom panels). Two unique experiments were performed. (c) Wild-type or FIP200 -/- MEF were incubated under nutrient rich, amino acid deprived or Torin-1 (+T, an mTOR inhibitor) conditions. WebNational Center for Biotechnology Information

Anti-ULK1 (S757) Antibody - Bosterbio

WebJun 16, 2024 · f Representative immunoblot and quantification of pULK1 S757 expression treated with CCCP (10 μM) for 24 h. g, h Representative immunoblot of p62 expression after the treatment as in b and quantification of basal levels. i Quantification of p62 degradation ratio as defined in e. WebJan 1, 2024 · Our results demonstrate two opposing events in the autophagic pathway: upregulation of the initiation of autophagy, suggested by the increase in pAMPK T172 and LC3 II, along with the decrease in pULK1 S757 at “early” timepoints (retinas of 5-month-old and 8 months old mice); downregulation of autophagy at the “later” timepoint (retinas ... pink wide leg cargo pants

Amino Acids Regulate mTORC1 by an Obligate Two-step …

WebpULK1(S757)(CS6888),rabbitanti-mTOR(CS2972),andrab-bit anti-phospho-mTOR (S2448) (CS2971S) from Cell Signal-ingTechnologyandmouseanti- -actin(sc-47778)fromSanta Cruz Biotechnology. Secondary antibodies were donkey anti-rabbit IRDye 680RD and donkey anti-mouse IRDye 800CW from LiCor. Blots were stripped between incubations using WebNov 12, 2024 · PVT elevates ULK1 mediated autophagy in PDAC cells. Western blot analysis of pULK1(S757), ULK1, Atg101, FIP200, Beclin-1, Atg5, and LC3A/B in (A) PO2 (B) BxPC3 (C) L3.6pl, (D) AsPC1, and (F ... WebpULK1-S555. Data are shown as mean phosphorylation (arbitrary units) ± s.e.m.; n = 3. Statistical significance vs. vehicle-treated WT was calculated by one-way ANOVA with Dunnett’s multiple-comparisons test. Representative immunoblots are shown. C) HEK293T cells expressing FLAG-tagged a1b1g1 or a2b1g1 were treated with 250 nM rapamycin or pink wide fit shoes and bag

Frontiers Deciphering the Mechanism of Bushen Huoxue …

Pulk1 s757

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WebMar 15, 2024 · Representative western blotting for autophagy-related genes (LC3II, P62, ATG7, Beclin-1, pmTORC1 S2448, pULK1 S757 and pAMPK T172) and the reference protein, GAPDH is shown (A,C,E). WebAug 28, 2024 · The densities of p-ATG13, p-BECN1, and p-ULK1 S757 are quantified and showed in the right panel. f HeLa cells were treated with different concentrations of PKC …

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WebFurthermore, we monitored increased levels of pp70S6K (T389), pULK1 (S757), and pAKT (S473), suggesting increased mTOR signaling ( Figure 5K, Figure S5U, S5V). ... View in full-text Context 19 WebJun 29, 2024 · Detection of pULK1 (S757) (A) or LC3-I/LC3-II (B) in B cells infected with indicated Salmonella strains for 3 h. ( C ) CFUs recovered from B cells infected with Salmonella strains for 24 h and treated for 1.5 h with autophagy-inducer peptide D11(20 μM) or scramble peptide L11 (20 μM).

WebPolyclonal Antibody for studying ULK1 (Ser758) phosphate. Cited in 342 publications. Validated for Western Blotting. Available in 2 sizes. Highly … WebFeb 1, 2024 · Western blot analysis exhibits the effect of Alda-1 on the phosphorylation of ULK1 with high glucose exposure. G: Representative gel blots of pULK1and β-actin (used as loading control) with specific antibodies; H:ULK1-to-β-actin;I: pULK1-s757-to β-actin;J: pULK1-s555-to β-actin;K: pULK1-s317-toβ-actin.All proteins were normalized to β-actin.

WebWestern blot analysis of extracts from various cell lines using Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb. Confocal immunofluorescent analysis of MCF7 cells, untreated … WebSep 17, 2024 · Interestingly, ULK1 S757 phosphorylation was not only maintained, ... (Figs. 2 and 6), signs of an mTORC1-dependent autophagy deficiency such as pULK1 S757 might also signal metabolic susceptibility.

WebJan 24, 2024 · Besides, pAMPK T172 and pULK1 S556 were decreased, and pmTOR S2448 and pULK1 S757 were increased. Cell experiments showed that the level of …

WebDec 6, 2024 · Reactivation of mTORC1 in full medium within 1 h was observed by phosphorylation of downstream substrates pULK1 (S757), pWIPI2 (S413), and pAMBRA1 (S52), ... Interestingly, although we observed reduced ULK1 S757 phosphorylation in USP32 KO cells and also accumulation of nuclear TFEB as a consequence of reduced mTORC1 … stein and terrace resturantWebMay 19, 2013 · Whole-cell lysates were immunoblotted with pULK1 S757 (mTORC1-target site), pS6K and ULK1 antibodies (bottom panels). Two unique experiments were performed. pink wig falmouth 2022WebFeb 13, 2024 · Primary antibodies against Met, pMet (Y1234/1235), p62, LC3, mTOR, pmTOR (S2448), pULK1 (S757), Atg5, Beclin-1, NBR1, and secondary horseradish peroxidase-conjugated goat anti-rabbit and anti ... pink wide legged trousersWebSep 17, 2024 · Interestingly, ULK1 S757 phosphorylation was not only maintained, ... (Figs. 2 and 6), signs of an mTORC1-dependent autophagy deficiency such as pULK1 S757 … pink wide width shoesWebUlk1 is phosphorylated at S757, while mTOR kinase activity is partially suppressed under hyperosmotic stress. (A) Wild-type MEFs and T24 cells were cultured with amino acid … pink wig for girlsWebPulk1 S757, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more pink wiffle ball batWebAug 11, 2015 · pULK1 (S757) pS6K (T389) S6K Rictor pRictor (T1135) pS6 (S235/236) B-- - MK PF R AS160 Akt pathway mTORC2 pathway mTORC1/S6K pathway C pSIN1 (T86) pAkt (T308) pS6 (S235/236) pNDRG1 (T346) 14-3-3 pPRAS40 (T246) Insulin Inhibitor PRAS40 Protor mTORC1 mTOR Raptor mLST8 Growth Protein Synthesis S6K … pink wide twill trousers